Lenti RT Activity Kit

High sensitive RT assay optimized for HIV-1, HIV-2, SIV, FIV and Visnavirus retroviruses

Analyze the activity of the enzyme reverse transcriptase (RT) from lentivirus with this kit. It can be used for quantification or screening for any lentivirus (HIV-1, HIV-2, SIV, FIV and Visnavirus) RT activity in cell culture supernatants.

Another application is screening for potential RT-inhibiting substances, either directly toward RT or in viral cell culture. The Lenti RT Activity Kit is not intended for use as a screening test for HIV-1 infection in humans nor is it to be used as a diagnostic test to confirm the presence of HIV-1 infection in humans.

Key Features: based on a highly sensitive, non-radioactive ELISA-technique using 96-well microtitre plate format (2 plates included in the kit)

Sensitivity: 1 to 2000 pg RT/ml

Read more in the Lenti RT Activity Kit instructions for use.

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CITATIONS AND REFERENCES

Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4+ T-cells.

Authors: Chan CH, McMonagle EL, Hosie MJ, Willett BJ

Journal: Virus Research. 2013, 171, 121–128

Product usage: Samples were stored at −80 °C until the end of the experiment at which time virus levels in the culture supernatants were measured by [...] a non-isotopic reverse transcriptase activity assay (Lenti RT kit, Cavidi AB, Sweden) as per manufacturer’s instructions

Human serum protein enhances HIV-1 replication and up-regulates the transcription factor AP-1.

Authors: Perdomo MF, Hosia W, Jejcic A, Corthals GL, Vahlne A.

Journal: PNAS. 2012, 109(43), 17639–17644

Product usage: PBMC: 200,000 freshly isolated, phytohemagglutinine-stimulated PBMCs were infected with 200 TCID50 of the primary isolate and cultured with human plasma and 200 IU interleukin-2. Control wells were cultured in 10% FBS. All samples were measured in quadruplicates in the same plate and in three different plates. RT was quantified at days 7 and 11 postinfection using the RT activity kit (Cavidi).

 

To read more, download our reference list from the menu on the left. Please feel free to contact us for more information.